diluted mouse anti human cx43  (Cell Signaling Technology Inc)

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Reduced expressions of connexin 43 and VEGF in the first-trimester tissues from women with recurrent pregnancy loss

doi: 10.1186/s12958-016-0179-4

Figure Lengend Snippet: Sequences of primers used for qRT-PCR analysis

Article Snippet: Sections were incubated with appropriate blocking serum (VECTASTAIN UNIVERSAL ABC kit, VECTOR) at room temperature for 20 min followed by incubation with diluted mouse anti human Cx43 (Cell Signaling Technology) or rabbit anti human VEGF (Abcam) overnight in a humidified chamber at 4 °C.

Techniques:

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Reduced expressions of connexin 43 and VEGF in the first-trimester tissues from women with recurrent pregnancy loss

doi: 10.1186/s12958-016-0179-4

Figure Lengend Snippet: The expression of Cx43 in chorionic villi and decidua collected from RPL women and control group. a , b The protein expression of Cx43 was measured by immunohistochemistry (magnification X400) and evaluated by IRS scores. The control group exhibited a higher level of Cx43 protein compared to the RPL group. c , d The Cx43 protein expression was assessed by Western Blot and the band intensity was evaluated. The protein level of Cx43 is significantly higher in the control group than that in the RPL group. e The expression of Cx43 mRNA was reduced in both villi and decidua from the RPL group and those from the control group. Data were presented as mean ± standard deviation (SD). *: P < 0.05; ** P < 0.01; ***: P < 0.001

Article Snippet: Sections were incubated with appropriate blocking serum (VECTASTAIN UNIVERSAL ABC kit, VECTOR) at room temperature for 20 min followed by incubation with diluted mouse anti human Cx43 (Cell Signaling Technology) or rabbit anti human VEGF (Abcam) overnight in a humidified chamber at 4 °C.

Techniques: Expressing, Immunohistochemistry, Western Blot, Standard Deviation

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Reduced expressions of connexin 43 and VEGF in the first-trimester tissues from women with recurrent pregnancy loss

doi: 10.1186/s12958-016-0179-4

Figure Lengend Snippet: Relationship of Cx43 and VEGF expressed in the chorionic villi collected from RPL women versus controls. The protein levels of Cx43 and VEGF expressed in the chorionic villi were positively and linearly related as revealed by western blot in both RPL group ( a ) and control group ( b ), though the immunohistochemical result did not find any correlation in either group (c, d ). The same positive and linear relationship was detected in the mRNA expression in the RPL group ( e ), but not in the control group ( f )

Article Snippet: Sections were incubated with appropriate blocking serum (VECTASTAIN UNIVERSAL ABC kit, VECTOR) at room temperature for 20 min followed by incubation with diluted mouse anti human Cx43 (Cell Signaling Technology) or rabbit anti human VEGF (Abcam) overnight in a humidified chamber at 4 °C.

Techniques: Western Blot, Immunohistochemical staining, Expressing

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Reduced expressions of connexin 43 and VEGF in the first-trimester tissues from women with recurrent pregnancy loss

doi: 10.1186/s12958-016-0179-4

Figure Lengend Snippet: Relationship of Cx43 and VEGF expressed in the decidua collected from RPL women versus controls. In the decidua, there was no significant correlation of protein expression of Cx43 and VEGF from RPL women ( a , c ) or the control group ( b , d ). This is consistent with the results of mRNA expression in both groups ( e , f )

Article Snippet: Sections were incubated with appropriate blocking serum (VECTASTAIN UNIVERSAL ABC kit, VECTOR) at room temperature for 20 min followed by incubation with diluted mouse anti human Cx43 (Cell Signaling Technology) or rabbit anti human VEGF (Abcam) overnight in a humidified chamber at 4 °C.

Techniques: Expressing